Applications of New Technologies to Classical Restriction Enzyme Labs
Tested Studies in Laboratory Teaching, 2016, Volume 37
Boriana Marintcheva
Abstract
Teaching labs employing restriction enzymes are a popular choice across the undergraduate curriculum.
Long waiting periods and ethidium bromide driven safety precautions are common factors limiting the
opportunities for a truly independent student experience. Recent technological advancements allow
improvements of classical labs by introducing high fidelity enzymes, new generation safer DNA stains, and
alternative DNA electrophoresis buffer with slower electrolyte exhaustion. The described approach can be applied to any existing restriction enzyme lab with minimal revision of lab materials as long as fast cutting versions of the used enzymes are available.
Keywords: agarose gel electrophoresis, restriction digest, ethidium bromide alternatives
Boston University (2015)
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