Modular Cloning and Sequencing: A Six-Week Project in Molecular Biology and Bioinformatics
Tested Studies in Laboratory Teaching, 2009, Volume 30
Dave L. Robinson, Joann M. Lau, Sandra Porter, Byrony S. Wiseman & Melissa Woodrow
Abstract
This project involves isolating (cloning) and analyzing a major portion of the gene for the enzyme Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from an uncharacterized plant species. This gene is considered a housekeeping gene (a continually-transcribed gene whose product is involved in basic cell function). It codes for an enzyme that catalyzes an important step of glycolysis, a stage of respiration that occurs in all living cells. Because of the central importance of GAPDH, this gene occurs in all plants, as well as in all other organisms.
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