Gene Expression Patterns in Drosophila Embryos Using lacZ Transgenes?

 

Catherine Silver Key, Julie Gates, Jessica K. Sawyer, & Kirsten A. Guss

Tested Studies in Laboratory Teaching, 2015, Volume 36

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Abstract

To appreciate that genes are temporally and spatially expressed, this hands-on laboratory allows students to observe patterns of gene expression in Drosophila embryos. Students groups collect embryos containing one of eight possible enhancers driving expression of lacZ. Subsequently, students run a colorimetric assay to discover the pattern gene expression which is then documented by microscopy. When the beta-galactosidase protein is expressed from the enhancer-lacZ transgene, the location of this enzyme can be detected by the catalysis of a colorless substrate into a bluish-purple colored product. Students submit reports identifying which enhancer drove lacZ expression in their unknown transgenic embryo(s).

Keywords:  Drosophila, embryo, lac Z reporter gene, beta galactosidase, gene expression, transgenes, developmental patterning, enhancer

University of Oregon (2014)