Using the Crystal Violet Biofilm Assay to Assess Efficacy of Traditional Family Remedies of Students

 

Jill Callahan

Tested Studies in Laboratory Teaching, 2016, Volume 37

Abstract

The majority of bacterial infections are not caused by free-living species, but rather surface-associated biofilms. Within such infections, levels of antibiotic resistance are rapidly on the rise, thus increasing the need to explore alternative remedies. This project examines how the semi-quantitative Standard Crystal Violet Biofilm Assay (adapted from O’Toole, 1998) can be used to assess the anti-biofilm properties of treatment agents tested. The model organism used in the study is Streptococcus mutans, a cariogenic species found in dental plaque. Remedies we have examined have been selected by students enrolled in BI498, research methods, based on their cultural backgrounds. Test agents have included miswak chew sticks, propolis, and green tea. Allowing students to combine their own treatments based on family traditions with antibiotic alternative research has served as an invaluable method of student engagement.

Keywords:  dental plaque, biofilm quantification, plant remedies

Boston University (2015)