Modular Approaches to Introducing CRISPR/Cas9 Genome Engineering Technology into Biology Labs

 

Michael J. Wolyniak & Anil K. Challa

Tested Studies in Laboratory Teaching, 2019, Volume 40

Abstract

CRISPR-Cas9 system as a genetic tool has emerged as one of the most important advances in the past 20 years. As this technology is currently making and will continue to make significant impacts in research across the life sciences, it is imperative that undergraduates become conversant in the workings of CRISPR-Cas9 system. In this workshop, we present how the CRISPR-Cas9 system can be introduced in a variety of modules using bioinformatics, in vitro studies and in vivo analysis (with zebrafish as one of the models) to develop gene knockouts that can be used for a variety of research questions. Using a workflow developed largely by undergraduate student researchers, we share our experiences with making CRISPR-Cas9 system accessible to undergraduate students at all levels. This workflow and experimental details can be effectively used to introduce, as well as strengthen fundamental ideas in the Central Dogma of Molecular Biology in addition to developing important basic skills in DNA science.

Keywords:  zebrafish, active learning, CRISPR/Cas9, central dogma of molecular biology

The Ohio State University (2018)