Using Allele-specific Primer PCR to Determine Human ABO Blood-type Genotypes

 

Eric S. Calhoun, Patrick Jungling, Kali B. Prillwicz, Irfan Khan, Senan Susan, Jason Papciak, & Michael P. Martin

Advances in Biology Laboratory Education, 2020, Volume 41

https://doi.org/10.37590/able.v41.art26

Abstract

Genotyping individuals has moved from the clinical laboratory with implications on human health to commercial application in order to examine ancestry as well as non-health orientated traits. In this four-week laboratory exercise, students use allele-specific primer PCR in order to determine their genotype at the ABO blood group locus. The five most common alleles are examined in order to determine the genotype of each sample. Additionally, students were asked to troubleshoot any unexpected results as well as re-design the protocols in order to perform a multiplex PCR.

Keywords:  PCR, ABO, Genetics, genotyping, Allele-specific primer

University of Ottawa (2019)