Tetrahymena is a freshwater, ciliated protozoan that is cheap, easy to maintain and well suited for students of all academic levels. It is an excellent model to gain experience with experimental design and to learn various laboratory techniques. The Tetrahymena lab has been used and modified in a core Cell & Molecular 2000 level course (~100 students) at Acadia University for the past 2 years. Every summer, Acadia runs a free, week-long science summer camp to girls in Grades 7 and 8. This laboratory will be one of the workshops offered in August 2019 and will include data analysis. ABLE Participants will study the rate of vacuole formation in Tetrahymena using a 5% India Ink solution and compound microscopes (100X or 400X). Participants will discuss the experimental setup based on variables to be tested, controls, limitations and how the lab can be adapted to address student outcomes in different courses. Discussion may also include maintenance of the Tetrahymena culture, creation of rubrics with an array of designs, student collaboration, and management of large data sets and how to make sense of it all.
Keywords: experimental design, phagocytosis, India Ink, Tetrahymena
University of Ottawa (2019)
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