The Hunt for MRSA and ESBLs: An Examination of Pathogenic Bacteria on a University Campus

 

Tara Halterman, Kaylee Brown, & Sarah E. Ruffell

Advances in Biology Laboratory Education, 2020, Volume 41

https://doi.org/10.37590/able.v41.art33

Abstract

The purpose of this laboratory exercise is to collect bacterial samples and identify Methicillin-resistance Staphylococcus aureus (MRSA) and bacteria containing Extended Spectrum Beta-Lactamases (ESBLs) on a university campus. In this workshop, participants will experience the laboratory exercise through a combination of hands-on activities, visual aids and discussions. Students formulate a hypothesis in regards to the presence of MRSA and ESBLs within campus buildings. Over the semester, students make growth media, collect and culture bacterial samples from door handles, and evaluate the bacteria present using morphology. The bacterial samples are collected from door handles, a fomite capable of harboring and allowing the spread of virulent bacteria. Students utilize skills such as aseptic techniques, microbiological culture media preparation, and quantifying bacteria using the standard plate count method. After the completion of the experiment, they analyze their data and create a resolution to the hypothesis they formulated.

Keywords:  microbiology, aseptic technique, laboratory exercise, hands-on learning, fomite, differential media, MRSA, ESBLs

University of Ottawa (2019)