Sister Chromatid Differentiation and Exchange in Chinese Hamster Ovary Cells in Culture

 

James Tan

Tested Studies in Laboratory Teaching, 1995, Volume 16

Abstract

This laboratory exercise is designed to illustrate the semi-conservative mode of DNA duplication through the expression of sister chromatid differentiation (SCD), the single-stranded nature of the eukaryotic chromosome organization, and the mutagenic effects of certain chemical or physical agents through induced sister chromatid exchange (SCE). Through individual work and/or a group project, students will learn the basic cell culturing and cytogenetic techniques for chromosome studies, especially the Fluorescence Plus Giemsa (FPG) technique for detecting SCD and SCE. Comprehensive background and technical information and notes for the instructor are included.

Keywords:  cell culture techniques, sister chromatid exchange, sister chromatid differentiation, Fluorescence Plus Giemsa technique, Chinese hamster ovary cells

Emory University (1994)