Association for Biology Laboratory Education

Cell Fractionation in Tetrahymena
 

Nancy J. Rosenbaum

Tested Studies in Laboratory Teaching, 1997, Volume 18

Abstract

To illustrate cell fractionation, nuclei are isolated from the ciliated protozoan, Tetrahymena thermophila. A table top clinical centrifuge is used for the fractionation steps and the procedure is monitored microscopically using a differential stain. To determine the efficiency of the procedure, cell and nuclear counts are determined with a hemacytometer. To quantify DNA, the Diphenylamine Reaction is carried out and the amount of DNA per nucleus is calculated.

Keywords:  nucleus, cell fractionation, hemacytometer, Tetrahymena thermophila

Boston University (1996)