Tested Studies in Laboratory Teaching, 1997, Volume 18
Abstract
To illustrate cell fractionation, nuclei are isolated from the ciliated protozoan, Tetrahymena thermophila. A table top clinical centrifuge is used for the fractionation steps and the procedure is monitored microscopically using a differential stain. To determine the efficiency of the procedure, cell and nuclear counts are determined with a hemacytometer. To quantify DNA, the Diphenylamine Reaction is carried out and the amount of DNA per nucleus is calculated.
We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok
