Clearing Techniques for the Study of Vascular Plant Tissues in Whole Structures and Thick Sections

 

J.M. Herr Jr.

Tested Studies in Laboratory Teaching, 1984, Volume 5

Abstract

The clearing technique presented here in 4 exercises changes the refractive properties of various organelles without altering the structural integrity of the cells. It effects a refractive uniformity among cellular organelles so that their distinctive and contrasting features can be revealed with phase contrast and Nomarski interference microscopy or through selective staining in conjunction with bright field optics.

Keywords:  clearing technique, clearing squash technique

Clemson University (1983)