DNA Profiling by Mutliplex PCR Amplification and Genotype Determination by Reverse Dot-Blot Hybridization to Sequence-Specific Oligonucleotide Probes

 

Ralph Rascati

Tested Studies in Laboratory Teaching, 2003, Volume 24

Abstract

Human DNA profiling has applications in paternity testing and forensics. This exercise provides students the opportunity to gain first-hand experience with procedures that are currently used to extract DNA from their own cells, quantify the DNA in the extract, perform a multiplex PCR amplification of several loci used in forensic analysis, and determine their own genotype at those loci. In addition, methods for analyzing results relative to existing population databases will be presented. The exercise is normally presented in the context of a laboratory course in Forensic DNA Analysis that presents students with a variety of techniques that have been and/or continue to be employed in forensic laboratories.

Keywords:  forensics, Dot-blot hybridization, human identity testing, DNA profiling, DNA fingerprinting, polymerase chain reaction

Louisiana State University (2002)