A Simulation of the Sanger (Dideoxy) Method for Sequencing DNA

 

Robert Kosinski

Tested Studies in Laboratory Teaching, 2003, Volume 24

Abstract

This paper describes a classroom role-playing exercise that simulates the Sanger method. A massparticipation version of the method uses from 6 to more than 100 students to play the role of nucleotides and can simulate the sequencing of DNA strands up to about 6 nucleotides long. An instructor demonstration version of the method can be used in classes of any size, and takes only a few minutes to complete. Both exercises emphasize that Sanger sequencing depends on random incorporation of ddNTPs in growing nucleotides.

Keywords:  DNA sequencing

Louisiana State University (2002)