Experimental Embryology: a modern approach to investigating the affects of Teratogens on Early Development

 

Brenda Judge Grubb

Tested Studies in Laboratory Teaching, 2007, Volume 28

Abstract

Hands-on laboratory experiences are a key component to undergraduate education. To fulfill this need, I have developed an experimental embryology lab course that introduces basic problems in developmental biology by direct experimentation. The effects of teratogens on development is an interesting area of study that allows the students to explore normal verses abnormal development. This also provides an opportunity for more advanced students to study how chemical exposure during critical times of development can alter gene expression. The course uses frogs (Xenopus laevis), chicks and zebrafish (Danio rerio) to demonstrate the usefulness of model systems in research. Both classical embryology and modern molecular manipulation of developing embryos are performed during the lab period, accompanied by an interactive lecture and discussion period. Students learn state of the art techniques by conducting experiments published in current research articles. The course invites both senior undergraduates and graduate students. Many first year graduate students take the class to learn techniques they will use in their research. Undergraduate students also work in the research lab on complementary experiments and help with preparations and observations outside of the class period.

Keywords:  embryology

Purdue University (2006)