A Methanol-Free Method of Extracting Secondary Chemicals
Jordan P. Sarver, Lawrence S. Blumer & Marius K. Denton
Tested Studies in Laboratory Teaching, 2008, Volume 29
Abstract
This experiment examined the inducible responses of tobacco. Leaf toxicity was assessed by a brine shrimp bioassay. A new method of extraction was used in this bioassay. Previously, secondary chemical were extracted from tobacco leaves using methanol. Our new method used a boiling 1% NaCl solution for secondary chemical extraction. This experiment was a test of this new methanol-free extraction method. Three concentrations of extracted secondary chemicals were evaluated by using volumes of boiling 1% NaCl extracts ranging from 1ml to 3ml. This new method is both safer and quicker than methanol extraction, and yielded very robust bioassay results.
Keywords: bioassay, plant-herbivore, plant toxin
University of Kentucky (2007)
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