Modular Cloning and Sequencing: A Six-Week Project in Molecular Biology and Bioinformatics

 

Dave L. Robinson, Joann M. Lau, Sandra Porter, Byrony S. Wiseman & Melissa Woodrow

Tested Studies in Laboratory Teaching, 2009, Volume 30

Abstract

This project involves isolating (cloning) and analyzing a major portion of the gene for the enzyme Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from an uncharacterized plant species. This gene is considered a housekeeping gene (a continually-transcribed gene whose product is involved in basic cell function). It codes for an enzyme that catalyzes an important step of glycolysis, a stage of respiration that occurs in all living cells. Because of the central importance of GAPDH, this gene occurs in all plants, as well as in all other organisms.

Keywords:  Molecular Biology, bioinformatics, polymerase chain reaction, GenBank

University of Toronto, Mississauga (2008)