Phenotypes to genotypes using C. elegans

 

Pliny A. Smith

Tested Studies in Laboratory Teaching, 2009, Volume 30

Abstract

This lab is a variation of the classic microbiology unknown identification labs; however, it uses the metazoan C. elegans and genetics. Students will be given a series of unidentified plates containing coded worm strains; known only to the instructor. By examining the phenotypes and other properties of the worms, the students will be instructed to identify the genotypes of each unknown from a chart of possible genotypes and phenotypes. Some of the unknowns cannot be distinguished from each other without additional testing, which the students should conduct to complete their project. These include tests for conditional alleles, dominance, and complementation of alleles. Deciding appropriate tests to conduct helps to develop the student’s ability to use available information and reagents in the design of a hypothesis.

Keywords:  Caenorhabditis elegans, phenotype, genotype

University of Toronto, Mississauga (2008)