Human Single Nucleotide Polymorphism (SNP) Determination

 

Deel, S., S. Singer, & D. Walser-Kuntz

Tested Studies in Laboratory Teaching, 2010, Volume 31

Abstract

SNPs are single nucleotide polymorphisms; they represent the simplest type of genetic variation between individuals. A SNP is a location in the genome where at least 1% of individuals in the population have a different nucleotide. In this laboratory exercise, students determine which allelic form of a particular SNP they have. Students isolate their own DNA, perform PCR to amplify a region surrounding this SNP, and use restriction fragment length polymorphism analysis to determine their genotype. This lab is suitable for introductory students and can illuminate PCR, gel electrophoresis, human genetics, and evolution (population data are available for this SNP).

Keywords:  genetics, human genetics, single nucleotide polymorphism (SNP), SNP

University of Delaware (2009)