Enzyme Exercises Designed for Both Laboratory and Internet Environments

 

Black, B. L., M. Niedzlek-Feaver, & M. J. Wingate

Tested Studies in Laboratory Teaching, 2010, Volume 31

Abstract

We have developed a flexible laboratory exercise that illustrates the principles of enzyme activity and can be used in introductory biology courses for biology majors or non-majors. Additionally, the exercise can be utilized in distance education to bring elements of the laboratory experience on-line. The exercise employs intestinal maltase to illustrate maltose hydrolysis via a colorimetric assay. The goals are to teach students about the properties of enzymes, including the accumulation of product over time, as well as the effects of pH, temperature, and non-competitive inhibition on enzyme activity. Additionally, students will learn skills in equipment use, data analysis and interpretation of graphs.

Keywords:  enzyme, enzymes, enzyme activity, maltase

University of Delaware (2009)