Plant Tissue Culture Techniques

 

Lorraine Mineo

Tested Studies in Laboratory Teaching, 1990, Volume 11

Abstract

Several tissue culture procedures, ranging from simple to more complex, are examined. These procedures can be performed by beginning and advanced biology students and include seed sterilization and aseptic seed germination, as well as techniques for callus proliferation, cell suspension culture, and anther culture. Choice of tissues, aseptic methods, media preparation, and experimental design are addressed.

Keywords:  callus, hormone, anther cultures, suspension cultures, totipotency

University of New Brunswick (1989)